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ABCB5 Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:2000, IHC-P: 1:200-1:500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human ABCB5. The exact sequence is proprietary. |
| Target | ABCB5 |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ABCB5 Mouse Monoclonal Antibody [orb499985]
IF, IHC-Fr, IHC-P, WB
Human
Mouse
Monoclonal
Unconjugated
200 μg, 200 μl, 50 μl, 100 μl

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Western blot analysis of ABCB5 expression in Hela (A), 293T (B) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 95 kD)

Immunohistochemical analysis of ABCB5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
ABCB5 Mouse Monoclonal Antibody (orb323085)
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