Anti-ABCA1

• Catalog Number: orb1140405
• Category: Antibodies
• Description: Mouse monoclonal antibody to ABCA1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Other
• Form/Appearance: Liquid
• Concentration: 100ug/100ul
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: Peptides amino acids 1800-2260 (C-Terminal end)
• Tested applications: ELISA, IHC, IP, WB
• Dilution range: Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
• Application notes: Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
• Antibody Type: Primary Antibody
• Clone Number: AB.H10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only