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Catalog Number | orb1140405 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to ABCA1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | AB.H10 |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human, Mouse, Other |
Isotype | IgG |
Immunogen | Peptides amino acids 1800-2260 (C-Terminal end) |
Antibody Type | Primary Antibody |
Concentration | 100ug/100ul |
Dilution range | Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 with 0.05% sodium azide. |
Note | For research use only |
Application notes | Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user. |
Expiration Date | 12 months from date of receipt. |