Anti-5T4 [5T4 (H8)]

• Catalog Number: orb2645818
• Category: Antibodies
• Description: Anti-5T4 [5T4 (H8)]
• Target: 5T4
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing male BALB/c mice with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM).
• UniProt ID: Q13641
• Tested applications: DOT, ELISA, IF, IHC, IP, RIA
• Clone Number: 5T4 (H8)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TPBG; M6P1; WAIF1; Trophoblast glycoprotein; 5T4 o
• Research Area: Cancer Biology
• Note: For research use only

Immunofluorescence staining of MCF7 cells with anti-5T4 antibody 5T4 (H8). Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of 5T4 (H8) (orb2645818) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645818, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.