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Anti-5T4 [5T4 (H8)]

SKU: orb2645818

Description

Anti-5T4 [5T4 (H8)]

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsDOT, ELISA, IF, IHC, IP, RIA
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG
Clone No.5T4 (H8)
ImmunogenThe original antibody was generated by immunizing male BALB/c mice with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM).
Target5T4
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TPBG; M6P1; WAIF1; Trophoblast glycoprotein; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; Wnt-activated inhibitory factor 1

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Anti-5T4 [5T4 (H8)]

Immunofluorescence staining of MCF7 cells with anti-5T4 antibody 5T4 (H8). Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of 5T4 (H8) (orb2645818) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645818, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
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DOT
Dot Blot
View Protocol
RIA
Radioimmunoassay
View Protocol

Anti-5T4 [5T4 (H8)] (orb2645818)

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Available Sizes

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0.2 mg
$ 600.00
Free Secondary Antibody (20 ul)0/0

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