Anti-14-3-3 epsilon Antibody

• Catalog Number: orb1423357
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 14-3-3 epsilon.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: YWHAE
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat, Virus, Zebrafish
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P62258
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 14-3-3 protein epsilon; 14-3-3E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of 14-3-3 epsilon staining in NIH3T3 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of 14-3-3 epsilon expression in HEK293T (A), Hela (B), H1688 (C), mouse liver (D), mouse testis (E), rat liver (F), rat testis (G) whole cell lysates.