Annexin VII/ANXA7 Antibody

• Catalog Number: orb106583
• Category: Antibodies
• Description: Annexin VII/ANXA7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VII (445-460aa TRSEIDLVQIKQMFAQ), different from the related mouse and rat sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 52739 MW
• UniProt ID: P20073
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Annexin A7;Annexin VII;Annexin-7;Synexin;ANXA7;ANX
• Note: For research use only
• Application notes: WB: The detection limit for ANXA7 is approximately 3ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC analysis of ANXA7 using anti-ANXA7 antibody. ANXA7 was detected in a paraffin-embedded section of Human Mammary Cancer tissue.

IF analysis of ANXA7 using anti- ANXA7 antibody.ANXA7 was detected in immunocytochemical section of A431 cells.

Western blot analysis of ANXA7 using anti-ANXA7 antibody.

Flow Cytometry analysis of HepG2 cells using anti-ANXA7 antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.