Annexin V/ANXA5 Antibody

• Catalog Number: orb182376
• Category: Antibodies
• Description: Annexin V/ANXA5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, - Immunofluorescence, 2μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Rat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35937 MW
• UniProt ID: P08758
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calpho
• Note: For research use only
• Application notes: WB: The detection limit for Annexin V is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Annexin V antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-Annexin V antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Annexin V using anti-Annexin V antibody.Lane 1:Rat Lung Tissue;2:Rat Brain Tissue;3:Rat Cardiac Muscle Tissue;4:Rat Kidney Tissue;5:HELA Cell;6:SMMC Cell;7:A549 Cell;8:SGC Cell.

IF analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in immunocytochemical section of RH35 cell.

IF analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in immunocytochemical section of U20S cell.

IF analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in paraffin-embedded section of human placenta cancer tissues.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in paraffin-embedded section of Mouse Kidney Tissue.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in paraffin-embedded section of Rat Kidney Tissue.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in frozen section of rat kidney tissues.

IHC analysis of Annexin V using anti-Annexin V antibody.Annexin V was detected in frozen section of human placenta tissues.