Annexin V-FITC Apoptosis Assay Kit

• Catalog Number: orb1409916
• Category: Tools
• Description: Detect apoptosis and membrane permability with the Annexin V-FITC Apoptosis Assay Kit. This in vitro apoptosis assay employs the green fluorescent Annexin V-FITC reagent to label exposed phosphatidylserine in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide. Analyze the fluorescent signals by flow cytometry.
• Target: Apoptosis, Exposed phosphatidyl serine
• Application notes: 1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH2O., 3. Reconstitute FLICA with 50 µL DMSO., 4. Dilute FLICA 1:5 by adding 200 µL PBS., 5. Add diluted FLICA to each sample at 1:30-1:60 (e.g. spike at 1:30 by adding 10 µL to 290 µL cultured cells)., 6. Incubate approximately 1 hour., 7. Wash and spin cells three times., 8. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope or flow cytometer. FLICA 660 excites at 660 nm and emits at 680-690 nm.
• Sample Types: Cell culture
• Storage: 2-8°C
• Note: For research use only