ANLN antibody

• Catalog Number: orb18593
• Category: Antibodies
• Description: Goat polyclonal antibody to ANLN
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Bovine, Human, Mouse
• Dilution range: ELISA: 1:64000, IHC-P: 3-10 μg/ml
• Conjugation: Unconjugated
• MW: 124; 120; 120
• Target: ANILLIN / Scraps (C Terminus)
• Entrez: 54443
• Protein Sequence: WQPDACYKPIGKP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ANLN antibody, anti Scraps antibody, anti ANI
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10ug/ml.WB: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Kidney using ANLN antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using ANLN antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using ANLN antibody