Angiotensinogen/AGT Antibody

• Catalog Number: orb389447
• Category: Antibodies
• Description: Angiotensinogen/AGT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115). Mouse Angiotensinogen shares 62.2% and 87.9% amino acid (aa) sequence identity with human and rat Angiotensinogen, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat Western blot, 0.1-0.5μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51990 MW
• UniProt ID: P11859
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Angiotensinogen;Serpin A8;Angiotensin-1;Angiotensi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Angiotensinogen using anti-Angiotensinogen antibody.Lane 1:mouse liver tissue;2:mouse spleen tissue;3:mouse testis tissue;4:mouse kidney tissue.

IHC analysis of Angiotensinogen using anti-Angiotensinogen antibody. Angiotensinogen was detected in paraffin-embedded section of mouse kidney tissues.