Angiotensin II Type 1 Receptor/AGTR1 Antibody

• Catalog Number: orb308765
• Category: Antibodies
• Description: Angiotensin II Type 1 Receptor/AGTR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AGTR1 (1-28aa MILNSSTEDGIKRIQDDCPKAGRHNYIF), different from the related mouse and rat sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 41061 MW
• UniProt ID: P30556
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Type-1 angiotensin II receptor;AT1AR;AT1BR;Angiote
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB:Lane 1:HEK293 cell;2:T-47D cell;3:PC-3 cell;4:U-87MG cell;5:HepG2 cell;6:COS-7 cell;7:rat brain tissue;8:rat liver tissue;9:rat kidney tissue;10:RH35 cell;11:mouse brain tissue;12:mouse liver tissue;13:mouse kidney tissue;14:mouse HEPA1-6 cell.

IHC analysis of AGTR1 using anti-AGTR1 antibody. AGTR1 was detected in a paraffin-embedded section of human mammary cancer tissue.