Angiopoietin-2/ANGPT2 Antibody

• Catalog Number: orb389452
• Category: Antibodies
• Description: Angiopoietin-2/ANGPT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Angiopoietin-2 recombinant protein (Position: E180-D283). Human Angiopoietin-2 shares 75% and 77.9% amino acid (aa) sequence identity with mouse and rat Angiopoietin-2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA(Cap) , 1-5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 56919 MW
• UniProt ID: O15123
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Angiopoietin-2;ANG-2;ANGPT2;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ANGPT2 using anti-ANGPT2 antibody.Lane 1:human HepG2 cell.

Sandwich ELISA - Recombinant human Angiopoietin-2 protein standard curve.Use in combination with reagents from Human Angiopoietin-2 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody. Angiopoietin-2 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody. Angiopoietin-2 was detected in paraffin-embedded section of human lung cancer tissues.