Angiogenin/ANG Antibody

• Catalog Number: orb389386
• Category: Antibodies
• Description: Angiogenin/ANG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human ELISA(Cap) , 1-5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 16550 MW
• UniProt ID: P03950
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNA
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Angiogenin/ANG antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody.Lane 1:human hepatocellular carcinoma tumor tissue (HCCT) lysates.

IF analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody and anti-Beta Tubulin antibody. Angiogenin/ANG was detected in immunocytochemical section of U2OS cell.

Sandwich ELISA - Recombinant human ANG protein standard curve.Use in combination with reagents from Human ANG ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody. Angiogenin/ANG was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue.

IHC analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody. Angiogenin/ANG was detected in a paraffin-embedded section of human lung cancer tissue.