Amyloid beta Antibody / APP

• Catalog Number: orb2644859
• Category: Antibodies
• Description: Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the /A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: APP/3345
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 578-680 from the human protein was used as the immunogen for the Amyloid Beta antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Amyloid Beta antibody is available for research use only.
• UniProt ID: P05067
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Amyloid Beta antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human U-87 MG cells with Amyloid Beta antibody (clone APP/3345, green) and Reddot nuclear stain (red).

IHC staining of FFPE human brain with Amyloid Beta antibody (clone APP/3345). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Amyloid Beta antibody (clone APP/3345). These results demonstrate the foremost specificity of the APP/3345 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Amyloid Beta antibody (clone APP/3345) as confirmation of integrity and purity.