Aminoallyl-dUTP-ATTO-647N

SKU: orb64153

Description

Aminoallyl-dUTP-ATTO647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO647N/dTTP ratio for Nick Translation: 30-50% Aminoallyl-dUTP-ATTO647N/50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1151.08 g/mol (free acid); Detected MW: 1150.40 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₅₄H₆₉N₆O₁₆P₃
• SMILES: P(=O)(OP(=O)(O)[O-])(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN(C(=O)c1c(C2=c3cc4c5=[N+](CCC4)CCCc5c3C(c3c2cc2c(c3)N(C(CC2C)(C)C)CC)(C)C)cccc1)C

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only