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Aminoallyl-dUTP-ATTO-550

Aminoallyl-dUTP-ATTO-550

Catalog Number: orb64161

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$ 750.00
Catalog Numberorb64161
CategoryTools
DescriptionAminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Transla-tion: 30-50% Aminoallyl-dUTP-ATTO550/50% dTTP.
Concentration1.0 mM-1.1 mM
Form/Appearancefiltered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5
Purity≥ 95% (HPLC)
MWTheoretical MW: 1098.97 g/mol (free acid); Detected MW: 1098.33 g/mol (free acid)
SMILES[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN(C(=O)c1c(C2=c3cc4C(CC([NH+]=c4cc3Oc3cc4NC(CC(c4cc23)C)(C)C)(C)C)C)cccc1)C)O
FormulaC49H61N6O17P3
Storagestore at -20 °C
NoteFor research use only
Application notesIncorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. < br>< b>Spectroscopic Propertie: λexc 554 nm, λem 576 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
Expiration Date12 months from date of receipt.