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Catalog Number | orb64161 |
---|---|
Category | Tools |
Description | Aminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Transla-tion: 30-50% Aminoallyl-dUTP-ATTO550/50% dTTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 1098.97 g/mol (free acid); Detected MW: 1098.33 g/mol (free acid) |
SMILES | [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN(C(=O)c1c(C2=c3cc4C(CC([NH+]=c4cc3Oc3cc4NC(CC(c4cc23)C)(C)C)(C)C)C)cccc1)C)O |
Formula | C49H61N6O17P3 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. < br>< b>Spectroscopic Propertie: λexc 554 nm, λem 576 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |