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AMIGO-1 Antibody: APC
Catalog Number: orb150472
| Catalog Number | orb150472 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against AMIGO-1 conjugated to APC |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | L86/33 (Formerly sold as S86-36) |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of mouse AMIGO-3 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | APC |
| MW | 60-80kDa |
| Target | AMIGO1 |
| Entrez | 229715 |
| UniProt ID | Q80ZD8 |
| NCBI | NP_001004293.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | AMIGO 1 antibody, AMIGO1 antibody, Adhesion molecu Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone L86/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) AMIGO-1 Antibody (D) Composite.
