AMFR Antibody

• Catalog Number: orb381041
• Category: Antibodies
• Description: AMFR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human AMFR recombinant protein (Position: E553-S643). Human AMFR shares 89% amino acid (aa) sequence identity with mouse AMFR.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 72996 MW
• UniProt ID: Q9UKV5
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase AMFR;6.3.2.-;Autocrine
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-AMFR antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-AMFR antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of AMFR using anti-AMFR antibody.Lane 1:human HepG2 cell; 2:human HeLa cell; 3:human Daudi cell; 4:rat kidney tissue.

IF analysis of AMFR using anti-AMFR antibody. AMFR was detected in an immunocytochemical section of T47D cells.

IHC analysis of AMFR using anti-AMFR antibody. AMFR was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of AMFR using anti-AMFR antibody.AMFR was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of AMFR using anti-AMFR antibody.AMFR was detected in paraffin-embedded section of mouse intestine tissues.