Alpha Tubulin Mouse Monoclonal Antibody

• Catalog Number: orb499957
• Category: Antibodies
• Description: Alpha Tubulin Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10F11
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Guinea pig, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: Recombinant human alpha Tubulin Protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:50000-200000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBA4A
• UniProt ID: P68366
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha tubulin 1; alpha-tubulin 1; Detyrosinated al
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of orb499957 10F11) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human A431 cell lysates, Lane 7: Human U251 cell lysates, Lane 8: Human 293T cell lysates, Lane 9: Human HepG2 cell lysates, Primary: Anti-alpha Tubulin (orb499957) at 1/100000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 50 kDa.