Alpha-synuclein/SNCA Antibody

• Catalog Number: orb1294391
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Synuclein
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:300 Immunohistochemistry (Frozen) 1:100-1:200
• Conjugation: Unconjugated
• Target: Alpha-synuclein / SNCA
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-alpha Synuclein/SNCA antibody - 1/700 dilution, Lysates at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294391 overnight at 4°C.

Immunofluorescent analysis. Sample: primary cortical neurons; alpha Synuclein (orb1294391): 1-400; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical of frozen sections. sample: mouse cerebellum. alpha Synuclein (orb1294391): 1-400; Anti-rabbit 488: 1-500.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294391 anti-alpha Synuclein antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.