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Alpha Synuclein (pSer129) Antibody

SKU: orb612713

Description

Rabbit monoclonal antibody to Alpha Synuclein (Phospho-Ser129)

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, ICC, IHC, WB
Dilution RangeWB (1:500)
ReactivityHuman, Mouse
Application Notes
A 1:500 dilution was sufficient for detection of Alpha Synuclein pSer129 in 10 µg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.J18
ImmunogenHuman alpha synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys
TargetAlpha Synuclein pSer129
PurificationAffinity Purified
ConjugationUnconjugated

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

4 Results
Dilution
Sample
Summary
IHC
Rat
1:200
Free-floating sagittal brain sections from adult Wistar rats
Sample preparation involved post-fixing brain tissue,...
Immunoblotting
Mouse
1:1000
Mouse brain
Homogenizing brain tissue in RIPA buffer,...
Immunostaining
Mouse
1:1000
Primary hippocampal neurons from C57/BL6 mouse embryos at the E16.5 stage
Cells were fixed with 4% paraformaldehyde/4%...
IHC
Human
-
Human peripheral blood smears and normal liver sections
Blood smears were fixed in absolute...

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Alpha Synuclein, α-Synuclein, SNCA, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Phosphorylated alpha synuclein, Phospho-alpha Synuclein (S129), Alpha-synuclein (phospho S129), Alpha Synuclein (phospho Ser129), Alpha-Synuclein Phospho Ser129, phospho-α-Synuclein (Ser129), Alpha Synuclein phospho Serine 129, Alpha Synuclein phospho Ser 129, Alpha Synuclein pSerine 129, Alpha Synuclein pSer 129, Alpha Synuclein phosphoSer 129, isoform NACP140, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4, Parkinson disease familial 1, SYN, Synuclein alpha, Synuclein alpha 140, Synuclein, alpha (non A4 component of amyloid precursor), SYUA_HUMAN

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Quality Guarantee

Quality Guarantee

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 770.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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