Alpha-internexin antibody

• Catalog Number: orb1473768
• Category: Antibodies
• Description: Mouse monoclonal antibody to Alpha-internexin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-internexin. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: INA
• Entrez: 226180, 9118
• UniProt ID: P46660, Q16352
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: NEF5; Alpha-internexin; Alpha-Inx; 66 kDa neurofil
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Alpha-internexin expression in SHSY5Y (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)

Immunohistochemical analysis of Alpha-internexin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Alpha-internexin staining in brain tissue cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).