Alpha Fetoprotein / AFP Antibody Cocktail

• Catalog Number: orb749480
• Category: Antibodies
• Description: It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant full-length human Alpha Fetoprotein was used as the immunogen for the AFP antibody cocktail.
• UniProt ID: P02771
• Tested applications: IF, IHC-P
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the AFP antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: C2 + C3 + MBS-12
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the AFP antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This AFP antibody cocktail is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human fetal liver stained with AFP antibody (C2 + C3 + MBS-12).