alpha 5 beta 1 Integrin Antibody [M200 (Volociximab)], Rabbit IgG - Research Grade Biosimilar

• Catalog Number: orb1671991
• Category: Antibodies
• Description: alpha 5 beta 1 Integrin Antibody [M200 (Volociximab)], Rabbit IgG - Research Grade Biosimilar
• Clonality: Recombinant
• Clone Number: M200 (Volociximab)
• Tested applications: Blocking, ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Heterodimeric α5β1Fc fusion protein
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: ITGA5
• UniProt ID: P08648
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fibronectin receptor, CD49 antigen-like family mem
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volocixim449). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of M200 (orb1671991) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671991- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volocixim449) PBMC's (A) and U93 cells (B) were stained with unimmunized r antibody (black line) or the rmeric version of M200 (Volocixim91 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.