Alpha-1-Trypsin antibody

• Catalog Number: orb344180
• Category: Antibodies
• Description: Alpha-1-Trypsin antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: a1-Anti-Trypsin [Human Plasma]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-ALPHA-1-ANTI-TRYPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P01009
• NCBI: NP_000286.3
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Alpha-1-Trypsin Antibody, A1AT antibody,
• Note: For research use only
• Application notes: Anti-Alpha-1-Anti-trypsin Antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of molecular weight (Lane1), Human transferrin (green) (Lane2) using Alpha-1-Trypsin antibody

Western blot analysis of molecular weight (Lane1), Goat-anti-Alpha-1-Anti-Trypsin (red) (Lane2) using Alpha-1-Trypsin antibody

Western blot analysis of Alpha-1anti-Trypsin under reducing conditions (Lane1), none (Lane2) Load: 1 ug per laneconjugated Donkey anti goat secondary antibody at 1:10