alpha 1 Fetoprotein/AFP Antibody

• Catalog Number: orb196260
• Category: Antibodies
• Description: alpha 1 Fetoprotein/AFP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 68678 MW
• UniProt ID: P02771
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetogl
• Note: For research use only
• Application notes: WB: The detection limit for AFP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of AFP using anti-AFP antibody.Lane 1:human HepG2 cell;2:human hepatocellular carcinoma tumor tissue (HCCT) lysates.

IHC analysis of AFP using anti-AFP antibody. AFP was detected in paraffin-embedded section of human liver cancer tissues.