Alpha 1 Antitrypsin Antibody / AAT / SERPINA1

• Catalog Number: orb750017
• Category: Antibodies
• Description: Alpha-1 Antitrypsin or a1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. Alpha 1-antitrypsin is also referred to as alpha-1 proteinase inhibitor (A1PI) because it inhibits a wide variety of proteases. It protects tissues from enzymes of inflammatory cells, especially neutrophil elastase, and has a reference range in blood of 1.5 - 3.5 gram/liter (in US the reference range is generally expressed as mg/dL or micromoles), but the concentration can rise manyfold upon acute inflammation. In its absence (such as in alpha 1-antitrypsin deficiency), neutrophil elastase is free to break down elastin, which contributes to the elasticity of the lungs, resulting in respiratory complications such as emphysema, or COPD (chronic obstructive pulmonary disease) in adults and cirrhosis in adults or children.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis). [Wiki]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity
• Immunogen: A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.
• UniProt ID: P01009
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: AAT/1379
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Alpha 1 Antitrypsin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Alpha 1 Antitrypsin antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1379). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1379). Confirmation of Integrity and Purity of the Antibody.