ALOX12 Antibody

• Catalog Number: orb1262471
• Category: Antibodies
• Description: ALOX12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 76 kDa
• Target: ALOX12
• UniProt ID: P18054
• NCBI: P18054
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 1
• Note: For research use only
• Application notes: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Fluor 554 (red) conjugated Phalloidin (red).

Western blot analysis of ALOX12 Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.