ALLO(Allopregnanolone) ELISA Kit

• Catalog Number: orb1946684
• Category: Assays and Kits
• Description: ALLO(Allopregnanolone) ELISA Kit
• Range: 1.57-100 ng/mL
• Target: AP
• Sensitivity: 0.55 ng/mL
• Alternative names: THP; 3a,5a-Tetrahydro Progesterone
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Allopregnanolone(ALLO) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Allopregnanolone(ALLO). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Allopregnanolone(ALLO) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.