Alkaline Phosphatase antibody (Peroxidase)

• Catalog Number: orb344858
• Category: Antibodies
• Description: Alkaline Phosphatase antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: E. coli
• Isotype: IgG
• Immunogen: Alkaline Phosphatase [E.coli]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against mammalian forms of Alkaline Phosphatase.
• Conjugation: HRP
• UniProt ID: P00634
• NCBI: NP_414917.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Alkaline Phosphatase Antibody Peroxida
• Note: For research use only
• Application notes: Anti-Alkaline phosphatase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Alkaline Phosphatase (E. Coli). Load: 50 ng per lane using Alkaline Phosphatase antibody (Peroxidase)