ALPL Antibody

SKU: orb1252202

Description

ALPL Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P
• Reactivity: Bovine, Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RTPrediluted format : incubate for 30 min at RT (1)Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: ALPL/597
• Immunogen: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.
• Target: ALPL
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALPL, Alkaline phosphomonoesterase, AP-TNAP, Alkaline phosphatase liver, APTNAP, Glycerophosphatase, Tissue-nonspecific ALP, TNAP, HOPS, Liver alkaline phosphatase, TNSALP

IHC testing of FFPE human colon carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human endometrial carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human testicular carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human ovarian carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

Gene Symbol

ALPL

UniProt

P05186