ALK Antibody / Anaplastic Lymphoma Kinase

• Catalog Number: orb2635788
• Category: Antibodies
• Description: The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ALK/1503
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human Anaplastic Lymphoma Kinase protein fragment (amino acids 1360-1460) was used as the immunogen for the Anaplastic Lymphoma Kinase antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Anaplastic Lymphoma Kinase antibody is available for research use only.
• UniProt ID: Q9UM73
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Anaplastic Lymphoma Kinase antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing (A) recombinant protein and (B) human HepG2 cell lysate using Anaplastic Lymphoma Kinase antibody (clone ALK/1503). Predicted molecular weight ~180 kDa but may be observed at higher molecular weights due to glycosylation. Caspase 3 induced cleavage results in an ~60 kDa intracellular ALK fragment.