Alginate Antibody

SKU: orb181872

Description

Mouse Anti-Alginate Monoclonal IgG1 Kappa

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:500), IHC-P (1:200)
• Reactivity: All
• Application Notes:
  1 µg/ml of SMC-208 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 4B10-1C5
• Immunogen: Sodium Alginate conjugated to KLH
• Target: Alginate
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Alginate, Sodium alginate, Calcium alginate, Alginic acid, Algin, Alginates, Alginic polysaccharide, Alginic acid sodium salt, Alginic acid calcium salt, Seaweed polysaccharide, Brown algae polysaccharide

Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:200.

Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.

Quick Database Links

Gene Symbol

Alginate