Alginate Antibody: Biotin

• Catalog Number: orb181901
• Category: Antibodies
• Description: Mouse monoclonal antibody against Alginate, conjugated to Biotin.
• Target: Alginate
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Biotin
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Immunogen: Sodium Alginate conjugated to KLH
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:500)
• Application notes: 1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 3G4-1F5
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Alginic Acid antibody, Algin antibody, Sodium Algi
• Note: For research use only

Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.