Aldolase antibody (Peroxidase)

• Catalog Number: orb344354
• Category: Antibodies
• Description: Aldolase antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human, Rabbit
• Isotype: IgG
• Immunogen: Aldolase [Rabbit Muscle]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].
• Conjugation: HRP
• UniProt ID: P00883
• NCBI: NP_001075707.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Aldolase Antibody, HRP conjugated goat a
• Note: For research use only
• Application notes: Anti-Aldolase has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 µg of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 300 ul aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls using Aldolase antibody (Peroxidase)

Immunoprecipitation analysis of was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts using Aldolase antibody (Peroxidase)

Western blot analysis of whole cell lysate prepared from human derived A293 cells using Aldolase antibody (Peroxidase)