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Catalog Number | orb18530 |
---|---|
Category | Antibodies |
Description | ALDH1A3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 56108 MW |
UniProt ID | P47895 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Aldehyde dehydrogenase family 1 member A3;1.2.1.5; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U-87MG cells using anti-ALDH1A3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ALDH1A3 using anti-ALDH1A3 antibody.Lane 1:rat kidney tissue;2:mouse gaster tissue;3:HEPG2 cell.
IF analysis of ALDH1A3 using anti-ALDH1A3 antibody.ALDH1A3 was detected in immunocytochemical section of A431 cell.
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in paraffin-embedded section of human gastric cancer tissues.
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in paraffin-embedded section of mouse kidney tissues.
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in paraffin-embedded section of rat kidney tissues.
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