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Catalog Number | orb371715 |
---|---|
Category | Antibodies |
Description | ALDH1A2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Predicted Reactivity | Bovine, Canine, Equine, Monkey, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ALDH1A2 recombinant protein (Position: M1-A110). Human ALDH1A2 shares 95.5% amino acid (aa) sequence identity with both mouse and rat ALDH1A2. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 56724 MW |
UniProt ID | O94788 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Retinal dehydrogenase 2;RALDH 2;RalDH2;1.2.1.36;Al Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of 293T cells using anti-ALDH1A2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ALDH1A2 using anti-ALDH1A2 antibody.Lane 1:human K562 cell; 2:human 293T cell; 3:human HepG2 cell; 4:human HeLa cell; 5:rat kidney tissue; 6:rat testis tissue; 7:mouse kidney tissue; 8:mouse testis tissue.
IHC analysis of ALDH1A2 using anti-ALDH1A2 antibody. ALDH1A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.
IHC analysis of ALDH1A2 using anti-ALDH1A2 antibody. ALDH1A2 was detected in a paraffin-embedded section of rat lung tissue.
IHC, IHC-P, WB | |
Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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