ALDH1A1 Antibody / Acetaldehyde dehydrogenase 1

• Catalog Number: orb2643241
• Category: Antibodies
• Description: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ALDH1A1/1381
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human recombinant partial ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ALDH1A1 antibody is available for research use only.
• UniProt ID: P00352
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human testicular carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human liver lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.

Western blot testing of human K562 (left) and lung (right) lysate with ALDH1A1 antibody (clone ALDH1A1/1381). Expected molecular weight ~55 kDa.

SDS-PAGE analysis of purified, BSA-free ALDH1A1 antibody (clone ALDH1A1/1381) as confirmation of integrity and purity.