ALDH1 Antibody

SKU: orb2642005

Description

ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  Titering of the ALDH1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: AHDH1-1
• Immunogen: A human recombinant partial ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human colon carcinoma with ALDH1 antibody (clone AHDH1-1).

IHC testing of FFPE human testicular carcinoma with ALDH1 antibody (clone AHDH1-1).

Western blot testing of human K562 cell lysate with ALDH1 antibody (clone AHDH1-1). Expected molecular weight ~55 kDa.

Quick Database Links

UniProt

P00352