ALDH18A1/P5CS Antibody

• Catalog Number: orb1522299
• Category: Antibodies
• Description: ALDH18A1/P5CS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 575 and 625
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ALDH18A1/P5CS
• UniProt ID: P54886
• NCBI: P54886.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: P5CS; Glutamyl-gamma-semialdehyde dehydrogenase; S
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ALDH18A1/P5CS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for IP at 6 µg per reaction.