Alcohol dehydrogenase class-3 ADH5 Antibody

• Catalog Number: orb381019
• Category: Antibodies
• Description: Alcohol dehydrogenase class-3 ADH5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39724 MW
• UniProt ID: P11766
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Alcohol dehydrogenase class-3;1.1.1.1;Alcohol dehy
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ADH5 using anti-ADH5 antibody.Lane 1:human HepG2 cell; 2:human K562 cell; 3:human MOLT4 cell; 4:rat liver tissue; 5:mouse liver tissue.

IF analysis of ADH5 using anti-ADH5 antibody.ADH5 was detected in immunocytochemical section of A431 cell.

IHC analysis of ADH5 using anti-ADH5 antibody.ADH5 was detected in paraffin-embedded section of human liver cancer tissues.

IHC analysis of ADH5 using anti-ADH5 antibody.ADH5 was detected in paraffin-embedded section of human mammary cancer tissues.