Albumin antibody (Biotin)

• Catalog Number: orb43650
• Category: Antibodies
• Description: Mouse Monoclonal to Albumin.
• Clonality: Monoclonal
• Clone Number: AL-01
• Tested applications: ELISA, IHC-P, RIA, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Positive control: human plasma, recommended antibody dilution: 2-5 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: Albumin
• Entrez: 213
• UniProt ID: P02768
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Albumin antibody
• Note: For research use only
• Application notes: Western blotting: Positive control: human plasma, recommended antibody dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human serum albumin using mouse monoclonal antibody AL-01 on human plasma and lysate of HeLa cell line (albumin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of biotinylated mouse anti-human albumin monoclonal antibody followed by IRDye800-conjugated streptavidin. Albumin was detected at approximately 50-67 kDa.