Albumin Antibody / Serum Albumin

SKU: orb607108

Description

Albumin is a soluble, monomeric protein, which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF, WB
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the Albumin antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: ALB/2356
• Immunogen: Recombinant human protein was used as the immunogen for this Albumin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of paraformaldehyde-fixed human HepG2 cells with Albumin antibody (green, clone ALB/2356) and Reddot nuclear stain (red).

Western blot testing of human HepG2 cell lysate with Albumin antibody (clone ALB/2356). Predicted molecular weigth ~66 kDa.

FACS testing of paraformaldehyde-fixed human HepG2 cells with Albumin antibody (blue, clone ALB/2356) and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Albumin antibody (clone ALB/2356). These results demonstrate the foremost specificity of the ALB/2356 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Albumin antibody (clone ALB/2356) as confirmation of integrity and purity.

Quick Database Links

UniProt

P02768