Alanine Transaminase antibody

• Catalog Number: orb345031
• Category: Antibodies
• Description: Alanine Transaminase antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Alanine Transaminase (ALT) [Pig Heart]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-ALANINE TRANSAMINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alanine Transaminase [Pig Heart]. Cross reactivity against Alanine Transaminase from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P13191
• NCBI: 113565
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-Alanine Transaminase Antibody, AAT1 ant
• Note: For research use only
• Application notes: Alanine Transaminase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Alanine Transaminase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.