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| Catalog Number | orb1173221 |
|---|---|
| Category | Assays and Kits |
| Description | Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit is designed for detecting ALT/GPT activity in the sample. |
| Hazard Information | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Storage | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt. |
| Alternative names | ALT; GPT; Alanine aminotransferase; glutamate pyru Read more... |
| Note | For research use only |
| Application notes | The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37°C and pH7.4 to produce glutamic acid and pyruvate; pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity. |
| Expiration Date | Please enquire. |
