Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit

SKU: orb1173221

Description

The kit provides a method for detecting ALT/GPT activity in biological samples including tissues, cell cultures, serum, and bacteria. ALT catalyzes the production of glutamic acid and pyruvate, which then reacts with 2,4-dinitrophenylhydrazine to form a brownish-red product.

Images & Validation

• Application Notes:
  The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37°C and pH7.4 to produce glutamic acid and pyruvate; pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.

Storage & Handling

• Storage: Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

ALT; GPT; Alanine aminotransferase; glutamate pyruvic transaminase