AKT3 Antibody (aa119-136, clone 66C1247.1)

• Catalog Number: orb1535719
• Category: Antibodies
• Description: AKT3 Antibody (aa119-136, clone 66C1247.1)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 66C1247.1
• Tested applications: IHC, IHC-P, WB
• Reactivity: Bat, Bovine, Canine, Equine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Isotype: IgG1
• Immunogen: A synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human AKT3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster (94%); Zebrafish (89%).
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: AKT3
• Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.05% Sodium Azide, 0.05% rAlbumin
• Alternative names: AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kin
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B1319 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1319 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.