AKT2 Western Chemiluminescent Blotting Kit Antibody

• Catalog Number: orb345940
• Category: Antibodies
• Description: AKT2 Western Chemiluminescent Blotting Kit
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 16G11.E8
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG2a
• Dilution range: WB: User Optimized
• Form/Appearance: n/a
• Purity: The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
• Conjugation: Unconjugated
• UniProt ID: P31751
• Storage: See kit insert for complete instructions.
• Buffer/Preservatives: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
• Alternative names: AKT 2 western blotting, AKT-2, AKT 2 antibody, AKT
• Note: For research use only
• Application notes: Use Biorbyt’ Anti-AKT2 Chemiluminescent Kit for Western Blotting for detection of human AKT2 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT2 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
• Expiration Date: Please enquire.

Western blot analysis of His-Tagged Recombinant (Lane1), His-Tagged Recombinant (Lane2), His-Tagged Recombinant Load: 50 ng per lane (Lane3) using AKT2 Western Chemiluminescent Blotting Kit