AKT2 Western Chemiluminescent Blotting Kit Antibody

SKU: orb345940

Description

AKT2 Western Chemiluminescent Blotting Kit

Images & Validation

• Tested Applications: WB
• Application Notes:
  Use Biorbyt’ Anti-AKT2 Chemiluminescent Kit for Western Blotting for detection of human AKT2 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT2 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Key Properties

• Reactivity: Human
• Conjugation: Unconjugated
• Purity: The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

AKT 2 western blotting, AKT-2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta, western bloting kit, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit

Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rat secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751