AKT2 Antibody (APC)

SKU: orb345019

Description

AKT2 APC antibody (APC)

Images & Validation

• Tested Applications: DOT, FC
• Dilution Range: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
• Reactivity: Human
• Application Notes:
  Anti-AKT2 APC Antibody is tested for Flow Cytometry. This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 11F6.B2
• Immunogen: Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
• Target: AKT2
• Purity: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein G chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
• Conjugation: APC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rat anti-Anti-AKT2 Allophycocyanin Conjugation, APC Conjugated rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Dot Blot of AKT2 Allophycocyanin Conjugated Monoclonal Antibody. Antigen: AKT2 non-phosphorylated. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: AKT2 Allophycocyanin Conjugated Monoclonal Antibody at 1:1000 in orb348637 for 60 min at RT. Block: orb348637 for 60 min at RT.

Flow Cytometry of Rat anti-AKT2 antibody. Cells: Jurkat Cells. Stimulation: none. Primary antibody: Allophycocyanin conjugated AKT2 antibody at 1.0 µg/ml for 20 min at 4°C.

Flow Cytometry of Rat anti-AKT2 antibody. Cells: SK-OV3 Cells. Stimulation: none. Primary antibody: Allophycocyanin conjugated AKT2 antibody at 1.0 µg/ml for 20 min at 4°C.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001617.1