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Description
Research Area
Cardiovascular Research, Metabolism Research, Protein Biochemistry, Signal Transduction
Images & Validation
−
| Tested Applications | FC, ICC, IF, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells |
| Reactivity | Human |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B32E8 |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Target | RAC-beta serine/threonine-protein kinase |
| Molecular Weight | 56 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Carrier-free |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt 2; Protein kinase B beta; RAC BETA; RAC PK beta
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Quick Database Links
Gene Symbol
RAC-beta serine/threonine-protein kinase
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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