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Item 1 of 5
AKT2 Antibody (Center)
Catalog Number: orb1929630
| Catalog Number | orb1929630 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB3026 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 55769 Da |
| Target | This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human AKT2. |
| UniProt ID | P31751 |
| NCBI | NP_001617.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | RAC-beta serine/threonine-protein kinase, Protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AKT2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
AKT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
AKT2 Antibody (N108) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).
Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).
