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Catalog Number | orb312153 |
---|---|
Category | Antibodies |
Description | AKT1(phospho-Ser129) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC-P, WB |
Predicted Reactivity | Canine, Equine, Gallus, Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser129 (DN(p-S)GA) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=2ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 56 kDa |
Target | phospho-AKT1 (Ser129) |
UniProt ID | P31749 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | AKT1 (phospho S129); p-AKT1 (phospho S129); AKT 1; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of A549.Primary Antibody (green line): Rabbit Anti-phospho-AKT1(Ser129) antibody; Dilution:2μg /10^6 cells.
ICC analysis of A549 cell using phospho-AKT1(Ser129) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Canine, Equine, Gallus, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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